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repetitive domestication to enhance butanol tolerance and

repetitive domestication to enhance butanol tolerance and

repetitive domestication to enhance butanol tolerance and

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Repetitive domestication to enhance butanol tolerance and ...Feb 01, 2013 · Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2 g/L to 15.3 g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increaseCited by: 51Publish Year: 2013Author: Xiao-Bo Liu, Qiu-Ya Gu, Xiao-Bin Yu

n-butanol: challenges and solutions for shifting natural repetitive domestication to enhance butanol tolerance and

Repetitive domestication to enhance butanol tolerance and production in Clostridiumacetobutylicum through artificial simulation of bio-evolution Bioresour Technol 2013The experimental evolution of herbicide resistance in repetitive domestication to enhance butanol tolerance andAug 22, 2012 · Xiao-Bo Liu, Qiu-Ya Gu, Xiao-Bin Yu, Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution, Bioresource Technology, 10.1016/j.biortech.2012.12.121, 130, (638-643), (2013).The Promising Fuel-Biobutanol | IntechOpenApr 28, 2012 · The increasing genetic knowledge provides feasible technique for the strain modification. Many efforts have been made to construct the strain with high butanol tolerance, superior butanol yield, productivity and less byproduct. The process can be classified into pathway-based construction and regulation-based construction.

Repetitive domestication to enhance butanol tolerance and repetitive domestication to enhance butanol tolerance and

Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2g/L to Cited by: 51Publish Year: 2013Author: Xiao-Bo Liu, Qiu-Ya Gu, Xiao-Bin YuRepetitive domestication to enhance butanol tolerance and repetitive domestication to enhance butanol tolerance andFeb 01, 2013 · Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2 g/L to 15.3 g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase Cited by: 51Publish Year: 2013Author: Xiao-Bo Liu, Qiu-Ya Gu, Xiao-Bin YuRepetitive domestication to enhance butanol tolerance and repetitive domestication to enhance butanol tolerance andRepetitive exposure and domestication of C. acetobutylicum T64 to increasing concentrations of butanol produced a mutant strain capable of withstanding 4% (v/v) butanol, whereas the wild-type repetitive domestication to enhance butanol tolerance and

Proteomic Analysis Identifies Dysregulated Proteins in repetitive domestication to enhance butanol tolerance and

Butanol can be produced biologically through fermentation of lignocellulosic biomass-derived sugars by Gram-positive Clostridium species. For cost-effective production, increased butanol fermentation titers are desired. However, the currently available butanol-fermenting microbes do not tolerate sufficiently high butanol concentrations; thus, new butanol-tolerant strains are desired. One repetitive domestication to enhance butanol tolerance andProgress and perspectives on improving butanol tolerance repetitive domestication to enhance butanol tolerance andFeb 11, 2017 · However, butanol toxicity to the producing microbes remains a barrier to achieving sufficiently high titers for cost-effective butanol fermentation and recovery. Investigations of the external stress of high butanol concentration on butanol-producing microbial strains will aid in developing improved microbes with increased tolerance to butanol.Cited by: 13Publish Year: 2017Author: Siqing Liu, Nasib Qureshi, Stephen R. HughesOVERCOMING CLONAL INTERFERENCE IN ESCHERICHIA 1.1 Two examples of arti cial adaptive evolution. A) Domestication of silver foxes. The initially feral foxes are selected for breeding based on their interactions with human handlers. Photo credits to Zefram and Dan Child (BBC). After a su cient number of generations, many of the foxes behave similarly to domesticated canines. B) Evolution

Novel distillation process for effective and stable repetitive domestication to enhance butanol tolerance and

Oct 20, 2018 · Yu X, Gu Q, Yu X. Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution. Bioresour Technol. 2013;130:63843. Article Google ScholarMetabolite labelling as a tool to define hierarchies in repetitive domestication to enhance butanol tolerance andsoils. In terms of butanol production, several mutants have been developed, both by random mutagenesis or engineered, that increase the butanol production of their parental strains. Usually, the butanol hyperproducing phenotype is obtained because the strain shows a higher tolerance to the solvent (butanol, Lin and Blas-chek, 1983; Liu et al repetitive domestication to enhance butanol tolerance andMetabolite labelling as a tool to define hierarchies in repetitive domestication to enhance butanol tolerance andIn terms of butanol production, several mutants have been developed, both by random mutagenesis or engineered, that increase the butanol production of their parental strains. Usually, the butanol hyperproducing phenotype is obtained because the strain shows a higher tolerance to the solvent (butanol, Lin and Blaschek, 1983 ; Liu et al ., 2013 ).

Low cell surface hydrophobicity is one of the key factors repetitive domestication to enhance butanol tolerance and

Nov 23, 2018 · The relative growth rate at 13% butanol (Figure 4B, C, and D) of the cultures with removed SLP demonstrated improved butanol tolerance, exactly correlating with CSH decrease. Regarding the strains Y22, S12, Ym1, and NCDO 1193, butanol resistance increase ranged from Improved n-butanol production via co-expression of repetitive domestication to enhance butanol tolerance andAmong them, the downregulated genes yghW and yibT were shown to improve n-butanol tolerance due to their regulatory roles in membrane fatty acid composition, while the upregulated genes gcl and glcF improved cell growth and metabolism by replenishing TCA cycle metabolic intermediates [ 38 ].Improved n-butanol production by a non-acetone producing repetitive domestication to enhance butanol tolerance andOne explanation for this may be the improved acclimation of C. pasteurianum cells to n-butanol stress in the presence of glucose, which resulted in a higher resistance to n-butanol toxicity. Indeed, the tolerance of the bacteria to higher n-butanol concentrations was significantly enhanced when both substrates were simultaneously fermented in anaerobic bottles compared to mono-substrate

GENOME ENGINEERING IN SACCHAROMYCES

biofuel. To enable this yeast as a producer for 1-butanol, which is a next-generation gasoline substitute, I discovered, characterized and engineered an endogenous 1-butanol pathway in S. cerevisiae. Upon introduction of a single gene deletion adh1, S. cerevisiae was able to accumulate more than 120 mg/L 1-butanol from glucose in the rich medium.Enhancing butanol tolerance and preventing degeneration in repetitive domestication to enhance butanol tolerance andOct 01, 2014 · In this study, 1-butanolglycerol storage was developed to enhance butanol tolerance and prevent productive degeneration in C. acetobutylicum. As a result, the optimal condition was using the BGS with 16 g L 1 butanol coupled with 200 g L 1 glycerol at 37 °C. Under this condition, C. acetobutylicum D64 preponderantly obtained an enhanced butanol tolerance of 32 g L 1 which was Cited by: 13Publish Year: 2014Author: Xiaobo Liu, Qiuya Gu, Chunli Liao, Xiaobin YuDomesticating brewing yeast for decreasing acetaldehyde repetitive domestication to enhance butanol tolerance andJan 29, 2014 · Liu XB, Gu QY, Yu XB (2012) Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution. Bioresour Technol 130(2):638643. Google Scholar

Development of optimal steam explosion pretreatment and repetitive domestication to enhance butanol tolerance and

Jun 24, 2020 · Second, a novel method integration of ARTP mutagenesis and repetitive domestication was applied for the generation of high furfural-tolerant microorganism. Finally, a highly effective conversion system of steam-exploded VR for butanol was established.Development of optimal steam explosion pretreatment and repetitive domestication to enhance butanol tolerance andInverse strategy employing ARTP and repetitive domestication for strain breeding is quite feasible, providing us with a new tool for solving the problem in the biofuel fields. Development of optimal steam explosion pretreatment and highly effective cell factory for bioconversion of grain vinegar residue to butanolDeveloping controllable hypermutable Clostridium cells repetitive domestication to enhance butanol tolerance andRepetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution. Bioresour Technol. 2013; 130 :638643. doi: 10.1016/j.biortech.2012.12.121.

Detection of subgenome bias using an anchored syntenic repetitive domestication to enhance butanol tolerance and

8 hours ago · Understanding the origin of this crop will help a wide range of researchers improve the breeding efforts and understand the process of crop domestication. The crop plant, E. coracana is believed to be the product of an allopolyploid hybridization between E. indica and another likely extinct species [8,9,10,11,12].Some results are removed in response to a notice of local law requirement. For more information, please see here.DOE Bioenergy Technologies Office (BETO) 2019 Project Redirect metabolism from native products to butanol Description of Risk Mitigation Plan Increasing alcohol concentration toxicity may be an issue for C. ljungdahlii Various in situ recovery methods exist to remove alcohol as it is being formed. Alternatively, butanol tolerance can be improved by genetic engineering Butanol may be not practical repetitive domestication to enhance butanol tolerance andChanges in Thermotolerance and Hsp70 Expression with repetitive domestication to enhance butanol tolerance andHere we use heat tolerance and expression of the inducible heat-shock protein, Hsp70, to examine phy-siological evolution and/or stasis in populations of Drosophila during laboratory domestication. We chose the rst trait because repetitive exposure to physiological stresses is sufcient for evolution of tolerance

10.1016/j.biortech.2012.12.121 | DeepDyve

Jun 11, 2020 · Butanol tolerance is a complex phenotype involving multiple loci ( Kevin and Smith, 2011; Papoutsakis, 2008 ), making the engineering of strains with enhanced tolerance to this solvent difficult. Hence, it is difficult to enhance butanol tolerance via gene engineering ( Dunlop et al., 2011 ).(PDF) Progress and perspectives on improving butanol Improvement in butanol tolerance sometimes leads to an increase in butanol production (42). The cellular responses and molecular mechanisms that can overcome butanol stress in Clostridium are repetitive domestication to enhance butanol tolerance and Repetitive domestication to enhance butanol repetitive domestication to enhance butanol tolerance andThrough repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2

A Study of Domestication and Foreignization repetitive domestication to enhance butanol tolerance and

Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution . 1 repetitive domestication to enhance butanol tolerance and

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