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development of a multiplex pcr assay for detection and

development of a multiplex pcr assay for detection and

development of a multiplex pcr assay for detection and

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Development of a Multiplex PCR Assay for Detection of ...Under the cold storage and processing conditions of raw milk, the psychrotrophic Pseudomonas fluorescens is usually found as predominant bacteria causing its spoilage. In this study, a multiplex PCR assay was developed for rapid and selective detection of P. fluorescens with biofilm formation ability. The target sequences were 2 genes (adnA and fliC) related to biofilm formation and flagella …Cited by: 4Publish Year: 2017Author: Yu Xu, Wanyi Chen, Chunping You, Zhenmin Liu

The development of a multiplex real-time PCR for the development of a multiplex pcr assay for detection and

A multiplex real-time PCR assay for rapid and simultaneous detection of HSV 1 and 2, VZV, adenovirus and Chlamydia trachomatis (C. trachomatis) from eye swabs was developed and evaluated. The multiplex assay was shown to be sensitive, specific and robust.Multiplex PCR: An overview of multiplex PCR assay, primer development of a multiplex pcr assay for detection andMultiplex PCR is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. In a multiplexing assay, more than one target sequence can be amplified by using multiple primer pairs in a reaction mixture.Development of two real-time multiplex PCR assays for The aim of this study was to develop and evaluate two multiplex real-time PCR assays for the sensitive detection and accurate quantication of Streptococcus pneumoniae, Haemophilus inuenzae, Staphylococcus aureus, Moraxella catarrhalis, Escherichia coli, Klebsiella

Development of multiplex PCR for neglected infectious

Jul 08, 2019 · The multiplex PCR is fast and time-saving because it is capable of detecting multiple pathogens at the same time [ 17 ]. In this study, we developed multiplex PCR to detect O. tsutsugamushi, L. interrogans, and R. typhi and evaluated its efficiency compared to serological tests.Cited by: 4Publish Year: 2019Author: Nutchanart Sea-liang, Amornpun Sereemaspun, Kanitha Patarakul, Jariyanart Gaywee, Wuttikon Rodkvamto development of a multiplex pcr assay for detection andDevelopment of multiplex PCR assay for simultaneous development of a multiplex pcr assay for detection andOct 30, 2014 · A multiplex PCR assay for simultaneous detection and discrimination of various Salmonella species in foodstuffs, poultry products and watershed samples has been developed and widely utilized for several types of conventional PCR methods (Hong et al. 2009; McCarthy et al. 2009; Park et al. 2009, 2011; Saeki et al. 2013). As multiplex PCR can development of a multiplex pcr assay for detection andDevelopment of a set of multiplex PCR assays for the development of a multiplex pcr assay for detection andJan 12, 2010 · To develop a rapid and reliable tool to detect by multiplex PCR assays the most frequently widespread -lactamase genes encoding the OXA-1-like broad-spectrum -lactamases, extended-spectrum -lactamases (ESBLs), plasmid-mediated AmpC -lactamases and class A, B and D carbapenemases.Cited by: 1218Publish Year: 2010Author: Caroline Dallenne, Anaelle Da Costa, Dominique Decré, Christine Favier, Guillaume Arlet

Development of a real-time multiplex PCR assay for the development of a multiplex pcr assay for detection and

Background. A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and Dublin.Development of a new multiplex quantitative realtime PCR development of a multiplex pcr assay for detection andMay 24, 2013 · Development of a new multiplex quantitative realtime PCR assay for the detection of the mtDNA 4977 deletion in coronary artery disease patients: A link with telomere shortening. Development of a new multiplex quantitative realtime PCR assay for the detection of the mtDNA. 4977.Development of a multiplex real-time PCR assay using two development of a multiplex pcr assay for detection andSep 21, 2018 · Development of a multiplex real-time PCR assay using two thermocycling platforms for detection of major bacterial pathogens associated with bovine respiratory disease complex from clinical samples John D. Loy, Laura Leger, Aspen M. Workman, Michael L. Clawson, Ece Bulut, and Bing Wang Journal of Veterinary Diagnostic Investigation201830:6, 837-847Cited by: 9Publish Year: 2018Author: John D. Loy, John D. Loy, Laura Leger, Laura Leger, Aspen M. Workman, Aspen M. Workman, Michael L. C development of a multiplex pcr assay for detection and

Development of a multiplex real-time PCR assay for the development of a multiplex pcr assay for detection and

T1 - Development of a multiplex real-time PCR assay for the detection of Bordetella pertussis and Bordetella parapertussis in a single tube reaction. AU - Arbefeville, Sophie. AU - Levi, Michael H. AU - Ferrieri, Patricia. PY - 2014/2/1. Y1 - 2014/2/1Cited by: 8Publish Year: 2014Author: Sophie Arbefeville, Michael H. Levi, Patricia FerrieriDevelopment of a multiplex real-time PCR assay for the development of a multiplex pcr assay for detection andJun 16, 2020 · Developed a rapid detection and quantification technique for Bacillus group-specific spp. and the genus Paenibacillus by using single quantitative PCR (qPCR) and multiplex quantitative PCR (mqPCR). The detection limit was 10 copy numbers.Cited by: 3Publish Year: 2020Author: Miyo NakanoDevelopment of a multiplex RT-PCR assay for the routine development of a multiplex pcr assay for detection andJul 01, 2020 · Multiplex RT-PCR detecting seven crucial bee viruses simultaneously were developed. The minimum detection amounts of the multiplex RT-PCR can reach to 10 4 copies/l. This newly developed assay is sensitive and specific. This method is Cited by: 1Publish Year: 2020Author: Abdurrahman Anil Cagirgan, Zafer Yazici

Development of a multiplex RT-PCR assay for simultaneous development of a multiplex pcr assay for detection and

Aug 22, 2019 · The detection methods of CGMMV or A. citrulli were widely reported, including one-step reverse transcription loop-mediated isothermal amplification for CGMMV detection (Li et al., 2013), multiplex RT-PCR assays for simultaneous detection of seven cucurbit-infecting viruses including CGMMV (Kwon et al., 2014), an ELISA-based assay for detection development of a multiplex pcr assay for detection andAuthor: Xinyue Bi, Xiaodong Li, Haibo Yu, Mengnan An, Rui Li, Zihao Xia, Yuanhua WuPublish Year: 2019Development of a multiplex PCR to detect and discriminate development of a multiplex pcr assay for detection andSep 05, 2019 · Three primer pairs were designed and used to develop a multiplex PCR assay. And 286 samples from 8 farms in Hubei province were tested by the developed multiplex PCR assay to demonstrate the accuracy. Each of target genes of PCV1, PCV2 and PCV3 was amplified using the designed primers, while no other porcine viruses genes were detected.Cited by: 3Publish Year: 2019Author: Keli Yang, Zuwu Jiao, Danna Zhou, Rui Guo, Zhengying Duan, Yongxiang TianDevelopment of a multiplex PCR assay for rapid and development of a multiplex pcr assay for detection andSpecies of genus Aeromonas, Vibrio, Edwardsiella and Streptococcus are the most common fish pathogenic bacteria that cause economically devastating losses in aquaculture. A multiplex polymerase chain reaction (mPCR) was developed for the simultaneous detection and differentiation of the four genera of fish pathogenic bacteria.

Development of a TaqMan Multiplex PCR Assay for

Nov 01, 2012 · Modifications to a previously designed endpoint AmpC multiplex PCR has allowed us to develop a real-time multiplex PCR assay using TaqMan probes for the detection of plasmid-mediated AmpC -lactamase genes, which allows ease of implementation into the clinical laboratory (9).Cited by: 16Publish Year: 2012Author: Chelsie N. Geyer, Mark D. Reisbig, Nancy D. HansonDevelopment of a New Multiplex Real-Time RT-PCR Assay Sep 19, 2020 · This research describes the development of a new multiplex real-time RT-PCR test for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with primers designed to amplify a 108 bp target on the spike surface glycoprotein (S gene) and a hydrolysis TaqMan probe designed to specifically detect SARS-CoV-2. The limit of detection (LOD) and clinical performance of Cited by: 2Publish Year: 2020Author: Wei Zhen, Gregory J. BerryDevelopment of a Multiplex Real-Time PCR Assay for the development of a multiplex pcr assay for detection andJun 01, 2012 · Due to several shortcomings to the assay, a multiplex real-time PCR assay (MRTA) to detect three ruminant species (bovine, caprine, and ovine) was developed using a lyophilized bead design. The assay contained two primer or probe sets: a ruminant set to detect bovine-, caprine-, and ovine-derived materials and a second set to serve as an development of a multiplex pcr assay for detection andCited by: 4Publish Year: 2012Author: Jason Ekins, Sharla M. Peters, Yolanda L. Jones, Heidi Swaim, Tai Ha, Fabio La Neve, Tiziana Civera, development of a multiplex pcr assay for detection and

Development of a Multiplex PCR Assay for Detection of development of a multiplex pcr assay for detection and

Under the cold storage and processing conditions of raw milk, the psychrotrophic Pseudomonas fluorescens is usually found as predominant bacteria causing its spoilage. In this study, a multiplex PCR assay was developed for rapid and selective detection of P. fluorescens with biofilm formation ability. The target sequences were 2 genes (adnA and fliC) related to biofilm formation and flagella Cited by: 4Publish Year: 2017Author: Yu Xu, Wanyi Chen, Chunping You, Zhenmin LiuDevelopment of a Multiplex PCR Assay for Detection and development of a multiplex pcr assay for detection andJan 01, 2012 · A multiplex PCR-based assay for N. meningitidis detection and genogrouping was developed based on screening out specific genes and primers for each N. meningitidis serogroup. This assay was tested on 179 bacterial isolates and proved to be specific, accurate, and sensitive.Cited by: 55Publish Year: 2012Author: Hongfei Zhu, Quan Wang, Liuqing Wen, Jianguo Xu, Zhujun Shao, Min Chen, Mingliang Chen, Peter R. Ree development of a multiplex pcr assay for detection andDevelopment of a Multiplex PCR Assay for Detection and development of a multiplex pcr assay for detection andArchana Vishwakarma, Gayathri Rethinavelu, Rathinsabapthi Pasupathi and Mohandass Ramya*Article: 6706 | Pages: Full Text

Development and validation of a multiplex, real-time RT development of a multiplex pcr assay for detection and

Development and validation of a multiplex, real-time RT PCR assay for the simultaneous detection of classical and African swine fever viruses. A single-step, multiplex, real-time polymerase chain reaction (RT-PCR) was developed for the simultaneous and differential laboratory diagnosis of Classical swine fever virus (CSFV) and African swine fever virus (ASFV) alongside an exogenous internal control RNA Cited by: 51Publish Year: 2013Author: Felicity J. Haines, Martin A. Hofmann, Donald P. King, Trevor W. Drew, Helen R. CrookeDevelopment and validation of a multiplex PCR assay for development of a multiplex pcr assay for detection andJun 26, 2018 · Summary We developed a multiplex PCR assay for the efficient detection of chicken, turkey and duck meat. Three speciesspecific primer sets targeting 12S rRNA gene of turkey and mitochondrial cytochrome b (CYTB) gene of chicken and duck were selected. A universal primer pair targeting 18S rRNA gene was additionally used as a positive control.Cited by: 4Publish Year: 2018Author: MiJu Kim, Insuk Yoo, SeungMin Yang, SeungMan Suh, HaeYeong KimDevelopment and evaluation of a panel of multiplex one development of a multiplex pcr assay for detection andJan 25, 2020 · A panel of locked nucleic acid based multiplex closed onetube nested realtime PCR (mOTNRTPCR) assay consisting of five separate internally controlled RTqPCR assays was developed for detection of 14 respiratory viruses.Author: Li Zhao, Guixia Li, Ji Wang, Mengchuan Zhao, Le Wang, Zhishan Feng, Xuejun MaPublish Year: 2020

Development and evaluation of a multiplex PCR assay for development of a multiplex pcr assay for detection and

Nov 03, 2010 · More recently, the use of multiplex PCR (m-PCR) has provided rapid and highly sensitive methods for the specific detection of pathogenic microorganisms in the aquatic environment. Furthermore m-PCR assay is more rapid and cost-effective than simplex PCR [3,10,13]. However, while m-PCR has been shown to be a valuable method for the identification of several pathogens in the field Cited by: 12Publish Year: 2010Author: Ertan Emek Onuk, Alper Ciftci, Arzu Findik, Yuksel DurmazDevelopment and evaluation of a multiplex PCR assay for development of a multiplex pcr assay for detection andMar 01, 1998 · Abstract. A multiplex polymerase chain reaction assay was developed for concurrent detection of rfb sequences specific for the O1 and the O139 serogroups of Vibrio cholerae and for ctxA specific sequences. The multiplex PCR assay was found to be highly specific and sensitive and was capable of detecting 65 cfu and 200 cfu per assay of V. cholerae O1 and O139, respectively.Cited by: 303Publish Year: 1998Author: Katsuaki Hoshino, Shinji Yamasaki, Asish K. Mukhopadhyay, Soumen Chakraborty, Arnab Basu, Sujit K. B development of a multiplex pcr assay for detection andDevelopment and Application of a Multiplex RT-PCR Assay development of a multiplex pcr assay for detection andA multiplex reverse transcription PCR assay for simultaneous detection of five tobacco viruses in tobacco plants. Journal of Virological Methods, 2012, 183(1): 57-62. [18]Li F, Zuo R, Abad J, Xu D, Bao G, Li R. Simultaneous detection and differentiation of four closely related sweet potato potyviruses by a multiplex one-step RT-PCR.

(PDF) Development of a Multiplex Real-Time PCR Assay for development of a multiplex pcr assay for detection and

Nov 26, 2020 · multip lex real- time PCR assay (MRTA) to detect three rumina nt speci es (bovine, caprine, and ovine) was developed using a lyophil ized bead design. The assay contained two (PDF) Development of a Multiplex PCR Assay for Detection development of a multiplex pcr assay for detection andNov 16, 2011 · A single-tube 5 nuclease multiplex PCR assay was developed on the ABI 7700 Sequence Detection System (TaqMan) for the detection of Neisseria meningitidis, Haemophilus influenzae, and development of a multiplex pcr assay for detection and

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